Abstract

Plant leaf starch content is a critical indicator of metabolic status, yet traditional enzymatic methods are destructive, labor-intensive, and costly. This study proposes a novel non-destructive detection method using watermelon–pumpkin grafted seedlings. To optimize hardware design, 12 characteristic wavelengths were identified via competitive adaptive reweighted sampling (CARS). A portable multispectral imaging system was developed, featuring narrowband LEDs and integrated human–computer interaction software for real-time visualization. We constructed a multimodal deep learning architecture that integrates a convolutional neural network (CNN) for spatial feature extraction from RGB images, a fully connected neural network (FCNN) for spectral data, and a Transformer network for high-level feature fusion. Experimental results showed that the ShuffleNet v2-Transformer model achieved an R2 of 0.956 (RMSE = 0.036) for watermelon leaves, while the EfficientNet b1-Transformer model reached an R2 of 0.967 (RMSE = 0.052) for pumpkin leaves. This multimodal approach significantly outperformed conventional PLSR and single-modal CNN models, demonstrating superior ability in processing long-range dependencies within spectral–spatial data. The system enables accurate detection with a throughput of 120 samples per hour at a hardware cost approximately 90% lower than commercial multispectral cameras. This provides an efficient, low-cost solution for large-scale monitoring of plant physiological indicators in precision breeding.

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