Abstract

This study investigated choline’s effects on endogenous phospholipid synthesis in Chinese mitten crab (Eriocheir sinensis). Chinese mitten crabs (0.40 g ± 0.03 g) were fed diets supplemented with 0%, 0.2% or 0.4% choline with low phospholipids (low-PL) or normal phospholipids (normal-PL) for eight weeks. Feeding the Chinese mitten crab with 0.4% choline in low-PL diets up-regulated significantly the relative mRNA expressions of neuropathy target enzyme 1 (nte1), phospholipase A2 (pla2) and phospholipase B (plb). Moreover, the crabs fed the 0.4% diet at low-PL diets enhanced hepatopancreatic fatty acid binding protein 3 (fabp3), fatty acid transporter protein 4 (fatp4), carnitine palmitoyltransferase-2 (cpt-2), carnitine acetyltransferase (caat), carnitine palmitoyltransferase-1a (cpt-1a) and carnitine palmitoyltransferase-1b (cpt-1b). However, feeding the mitten crabs 0.2% choline in the normal-PL diets diets up-regulated significantly the relative mRNA expressions of nte1, pla2 and plb. These results indicate that 0.4% choline up-regulates the expressions of genes involving in phospholipids synthesis of Chinese mitten crab fed with low-PL diets, while 0.2% choline improved the genes involved in phospholipid decomposition in normal-PL conditions.

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