Abstract

Environmental contamination represents an important risk factor for the transmission of healthcare-associated pathogens in both human and veterinary medicine. In equine hospitals, open stable environments and high patient turnover may further increase the risk of microbial spread. The aim of this study was to evaluate the hygiene status of an equine referral hospital and to assess the effect of implementing standardized cleaning and disinfection procedures combined with targeted staff training on environmental microbial burden. Surface samples were collected using agar contact plates from predefined environmental surfaces in surgical areas, examination rooms, and stable units of an equine clinic. Sampling was conducted during three investigation periods: prior to intervention, after implementation of revised cleaning protocols and staff training, and during a later follow-up assessment. Colony-forming units (CFU) were quantified and categorized into an ordinal contamination scale (0–4) to enable standardized statistical analysis. Furthermore, delta values were calculated for every sampling period, describing the reduction in the environmental microbial burden. Pre- and post-cleaning microbial burdens were compared using nonparametric statistical methods. A total of 76 locations were sampled before and after cleaning and disinfection in sampling period 1 and 2; in sampling period 3, sample collection focused on sensitive surfaces, and 21 locations were sampled. Bacterial contamination varied considerably between functional areas, with the highest baseline contamination consistently observed in stable environments. Examination and surgical areas showed heterogeneous contamination patterns, particularly on high-touch surfaces, such as keyboards, switches, and door handles. In sampling periods 1 and 2, cleaning and disinfection significantly reduced microbial burden. The delta values in sampling period 2 were significantly lower than the delta values in both sampling period 1 and sampling period 3. These findings demonstrate that environmental contamination in equine hospitals is highly variable and influenced by surface type, clinical workflow, and proximity to patients. Structured hygiene interventions, including standardized cleaning protocols and continuous staff education, can significantly improve environmental hygiene and should be integrated into infection prevention programs in veterinary clinical facilities.

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