Abstract

Background: Normalized strength outcomes and limb symmetry indices (LSIs) are widely used but poorly characterized across testing conditions, and it is unclear if these vary by sex. This study aimed, first, to investigate sex-related differences in normalized knee flexor strength and LSI values across multiple strength-testing conditions in healthy recreational athletes, and, second, to descriptively examine associations among strength outcomes obtained under different testing conditions within female and male participants. Methods: In this cross-sectional study, 52 healthy, recreationally active adults (26 females and 26 males) underwent bilateral knee flexor strength testing using three force plate-based isometric assessments, one static dynamometer-based isometric assessment, and three isokinetic dynamometer-based assessments. Differences were analyzed with a mixed analysis of variance (ANOVA), and associations were assessed using Pearson correlations. Results: Males showed higher normalized knee flexor strength than females across all testing conditions (main effect of sex: p < 0.001; partial η2 = 0.334–0.371), with the magnitude of these sex-related differences varying across testing conditions (sex-by-testing condition interaction: p < 0.001; partial η2 = 0.215–0.230). LSI values did not differ by sex (p = 0.896) and remained consistent across testing conditions (p = 0.385). Correlations were generally stronger within force plate-based and isokinetic dynamometer-based assessments (r = 0.528–0.922) than between different testing conditions. Conclusions: Sex-related differences were observed for normalized knee flexor strength but not for LSI values. Strength outcomes obtained under different testing conditions should not be considered directly interchangeable.

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