Abstract

The UBP gene family plays a key role in various physiological processes, including cell cycle regulation, DNA repair, and stress responses. UBPs have been identified and analyzed in several species, including Moso Bamboo, Arabidopsis thaliana, Oryza sativa L., and Brachypodium distachyon. However, the UBP family within Prunus mume Siebold & Zucc. remains unexplored. In this study, 30 PmUBPs were identified and characterized, all of which contained the UCH conserved domain. They were grouped into 16 subfamilies via evolutionary analysis, revealing similarities in genome structure and motif distribution. PmUBPs were distributed on seven chromosomes and three scaffolds. Gene duplication events, including tandem duplication and segmental duplication, promoted the expansion of PmUBPs. Collinear analysis demonstrated the close relationship between P. armeniaca, P. persica, P. avium, and P. mume. Transcriptome data revealed distinct relative expression trends in PmUBP genes across different tissues. Promoter cis-acting element analysis revealed an abundance of hormone-, light-, and stress-responsive elements across all genes. Transcriptome and reverse transcription quantitative PCR (RT-qPCR) analysis showed that PmUBP2, PmUBP11, PmUBP13, PmUBP18, PmUBP22, and PmUBP26 exhibited relatively high expressions under low-temperature stress. This study provides a theoretical foundation for the role of PmUBP genes in response to low-temperature stress.

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