In recent years, it has shown that real-world operating scenarios have a significant influence on the regulation and homologation of emissions and fuel consumption. Given the global efforts to reduce emissions of climate-relevant and polluting gases, a deep understanding of the real-world driving behavior of mobile sources is required. While emissions and fuel consumption for passenger cars under real-world conditions deviate considerably from officially published values, a methodology for measuring real vehicle system emissions (RDE) has not yet been adapted for motorcycles. This work describes an RDE measurement methodology for motorcycles conducted at the Graz University of Technology to prepare for potential future regulatory requirements. The collected data led to insights into motorcycle driving dynamics, which are integrated into the evaluation of real-world driving behavior. Furthermore, results and comparisons of emission measurements on the road and on test benches are presented to illustrate the relevance of various measurement methods. The goal is to gain a better understanding of the factors influencing RDE and to compare and evaluate the data with automotive applications. These findings are crucial for the development of more environmentally friendly mobility solutions for motorcycles.